Molecular basis of RNA-dependent RNA polymerase II activity Coursework

Molecular basis of RNA-dependent RNA polymerase II activity - Coursework Example The RdRP activity has also been implicated in the replication of the RNA Genomes of HDV virus and plant viriods. 3) What is meant by HDV? Why would investigators want to study HDV? In your answer, tell me about HDV and why investigators would want to study HDV. What is the ANTIGENOME? Why is it important in the experimental design? HDV is Hepatitis Delta Virus. The investigators have taken HDV for the study as RdRP activity of Pol II has been established to be involved in the replication of the viral genome. Antigenome is the complementary positive RNA which serves as the template for the synthesis of the negative strand of the virus. To understand the physiological significance of the RdRP activity, the terminal segment of the HDV antigenome, which directs RNA synthesis and is sensitive to the Pol II inhibitor alpha- amanitin, was studied. 4) What is meant by â€Å"FAM-labeled RNA products were separated by denaturing gel electrophoresis†? DO NOT give me cookbook recipes, i.e. do not provide a ‘step-by-step how to’ that could be used to perform an experiment. Describe the method in general terms. FAM is 6 carboxy- flourescein label. Nucleotides labeled with such flourophores, have shown improved sensitivity, versatility and normalization when separated by denaturing gel electrophoresis. After purification of the nucleotides the fluorescein tag was attached by incubation with the 5’ terminal of the nucleotides. 5) DATA INTERPRETATION: Evaluate Figure 1. Break the analysis down. Strive to understand what each lane of the gel represents. Make note of the controls as well. Your focus for your response is the part of the figure labeled b, c, and d. Use part a in assisting you. Summarize what each part (b, c, and d) of the figure suggests. The RNA synthesized by the RdRP activity of Pol II is fluorescent labeled and separated by gel electrophoresis and viewed. Lane 1 in the gel shows only the reactant RNA.

Class Movie Review Example | Topics and Well Written Essays - 750 words

Class - Movie Review Example The opening clips are really not surprising because the United States Secretary of Defense is always accusing the Iraqis of presenting a biased view of the American operation yet the Iraqi Minister of Information was doing the exact same thing. The way that the documentary shows these two opposing views side by side is making fun of them in a way because it shows how single minded some news organizations can be when they have already decided what the news will be and then they find a story to suit that. Other networks are shown to only select news stories that fit their point of view, while a producer for Al Jazeera comments that he wants his news channel to set the standard for news reporting in the Middle East. â€Å"Control Room† follows the careers of three main individuals: Josh Rushing, David Shuster, and Tom Mintier. All of these reporters are American and so they have instruction to only show the Iraq War in a certain light. Josh Rushing is perhaps the most interesting of these three reporters because he works at the U.S. Central Command, or what is known as CENTCOM. At the start, Rushing shows how much he dislikes the Al Jazeera network because it does not show any of crimes committed by Iraqi soldiers. An American reporter then claims that there is no footage of such events even though they are likely occurring. The problem is that without any evidence of these crimes, most Arab people had a hard time believing it. Rushing also says that many American news media are guilty of the same thing, except in the opposite way. The whole film is built around this central idea that news media organizations in general are bias and only show stories that fit their beliefs. The problem i s that the truth is not told, and this is a really important during the time of war. The film changes tact part way through and focuses on American